Comparative Effects of Cissus Populnea Extract and Jatropha Curcas on Propoxur-Induced Testicular Toxicity of Adult Male Wistar Rat

This study is aimed to compare the ameliorative properties of Cissus populinea and Jatropha curcas on testicular damage caused by propoxur. Eighteen adult male Wistar rats were used in this study and were divided into six groups A, B, C, D, E, F consisting three rats in each group. Group A received 1ml distilled water and served as the control group. Group B received 0.72 mg/liter/kg body weight of Propoxur. Group C received 200 mg/kg body weight of Jathropha curcas. Group D rats received 200 mg/kg body weight of Cissus populnea. Group E rats received 0.72 mg/liter/kg of propoxur and 200 mg/kg body weight of Jathropha curcas. Group F received 0.72 mg/liter/kg of Propoxur and 200 mg/kg body weight of Cissus populnea extract. The administration was done once in a day for 14 days. After which the rats were sacrificed and the testes were removed, weighed and fixed in 10% buffered formo-saline and processed using the Haematoxylin and Eosin staining technique for histological observations, sperm analysis were also carried out on the testes. Blood sample were also collected through cardiac puncture to evaluate for serum level of follicle stimulating hormone (FSH), leutenizing hormone (LH) and testosterone (TT). There was significant (p<0.05) decrease in body weight and testicular weight of animals in group B, however, no significant difference was observed in sperm count, but sperm motility and morphology was significantly increased in group B and F animals. FSH, LH and testosterone was significantly p<0.05 decrease in group B and C animals. There was mild destruction of testes in group B and C as evidence from histology sections while group D shows some regenerative effects. In conclusion, propoxur causes moderate destruction of the testes which is evident by progressive tissue damage while Cissus populnea had some regenerative effect and improve the testes however Jathropha curcas had mild destructive effect on the testes.

Key Words: Propoxur, Cissus populnea, Jathropha crucas, sperm parameters