Histological studies of ethanolic extract of Costusaferkergawl leaves on the pancreas of alloxan-induced diabetes in Wistar rats
The hypoglycaemic property of ethanolic leaves extract of Costusaferkergawl on the pancreas of alloxan induced diabetic Wistar rats was evaluated to unravel the basis for its use in diabetes. Twenty-five male wistar rats weighing 180-200g were equally distributed into five groups of five rats designated A, B, C, D and E. Group A served as the normal control and did not receive any treatment, groups B, C, D and E were fasted overnight and diabetes was induced using single dose of 140mg/kg alloxan monohydrate (i.p). Group B served as the diabetic control and did not receive any other treatment while groups C, D and E received 100, 200 and 300mg/kg body weightof ethanolic leaf extract ofCostusaferkergawl respectively for 14days. Blood was collected from tail vein for estimation of fasting blood glucose levelson the 0, 7 and 14thday of experiment. On day 15, the animals were anesthetized using 4mg/kg ketamine (i.p), the pancreas were harvested and processed for histological studies using H and E technique. Results of this study showed that ethanol extract of Costus afer ker gawl leaves ameliorated the diabetogenic effect of alloxan and decreased significantly the blood glucose level (P? 0.05) in treated rats when compared to the diabetic control (group B). Morphometric study of pancreas revealed that Costus afer ker gawl extract restored significantly the islets of Langerhans against alloxan-induced tissue changes. However, this restoration was dose dependant. In conclusion, ethanolic leaves extract of Costus afer ker gawl may have hypoglycaemic potentials.
Key Words: Diabetes, Costus afer ke rgawl, antidiabetes, pancreas, Wistar rats.